Facilities

ECOTOX research activities take place at the Molecular Laboratory located at CITEXVI (Lab 99, 2^nd floor) and at Toralla Marine Station (ECIMAT) integrated in the European Marine Biological Research Centre (EMBRC-ERIC).

Equipment available:

Own:

• Molecular biology:
  • DIGICEN 20R Refrigerated Centrifuge, ORTO ALRESA.
  • Molecular Imager Gel Doc™ XR+ System with Image Lab™ Software, BIO-RAD, automated image capture and analysis of gels and blots, 2 modes (trans-UV and epi-white).
  • Trans-Blot Turbo™ Transfer System, BIO-RAD: western blotting transfer system.
  • T100™ Thermal Cycler, BIO-RAD: nucleic acid amplification / make running PCR.
  • Mini-PROTEAN Tetra Handcast Systems, BIO-RAD: electrophoretic chamber used in polyacrylamide gel electrophoresis (PAGE or SDS-PAGE).
  • Wide Mini-Sub Cell GT Cell, BIO-RAD: electrophoretic chamber used in nucleic acid gel (Agarose).
  • PowerPac™ Basic power, BIO-RAD: power supply to horizontal and mini vertical gel electrophoresis (Nucleic acid gel electrophoresis / Protein gel electrophoresis).
• Leica DMI 4000B inverted microscope, Digital Camera Leica DFC 295, and Image Analysis Software LAS (Leica Application Suite).
• Polarographic Stand 663VA Methrom with Eco Chemie Autolab PGSTAT10 potentiostat and BASi potentiostat CGME with BAS CV-50W for the determination of metals in seawater.
• BOD Sensor VELP System 6 with incubator.
• Oximeter OXI 330i Crison; Salinometer w/ conductivity cell 013010-ATI-Orion; Metrohm 744 pH meter: water quality measurement equipments.
• Cryopreservation system of CryoLogic CONTROL SYSTEMS Pty. Ltd. for cryopreservation of biological material.

Shared:

• Sea water system (3000 L) to maintain stocks of marine invertebrates: Routine maintenance of adult stocks of echinoderms (Paracentrotus lividus) crustaceans (Acartia sp, Corophium sp.) or fish with the aim of obtaining biological material for embryolarval bioassays.
• Isothermal rooms for phytoplankton culture with routine cultures Isochrysis galbana, Tetraselmis suecica, Chaetoceros gracilis, Selenastrum capricornutum and other microalgal species used in bioassays.
• Nikon Microscopes: 90i fluorescence microscope, inverted microscope TE2000S, 50i Microscopes, Microscope Inverted Microscope Eclipse TS100 and E200.
• Nikon Magnifying: SMZ1500, SMZ1000, SMZ70 and SMZ800.
• Isothermal Rooms for embryolarval bioassays.
• Freezers at -20 °C and -80 °C.
• Stoves 80, INDELAB IDL-EI-208.
• Muffle Oven, Obersal 10 PR/400 PAD.
• Autoclave Raypa AES110.
• DirectQ-5, ultrapure water.
• Refrigerated Centrifuge Hettich Zentrifugen Rotanta 460 R.
• Beckman DU640 spectrophotometer.
• Freeze Dryer Christ Alpha 1-4.
• Particle Counter, Beckman Coulter Multisizer 3.
• Gas flumes.
• PH meter Crison GLR 21
• Oxyguard Handy Polaris
• Portable Oxymeter, Oxymeter YSI 500, Thermo-salinity meter Radiometer PIONEER 30.
• Membrane filtration system 0.22 mm, Millipore YT30 142 HW.
• Distiller Raypa DES 4.
• Magnetic stirrers, precision balances.

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